LOCUS       Exported                7247 bp ds-DNA     circular SYN 14-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAd1130-03
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 14, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      4446..4454
                     /regulatory_class="other"
                     /note="ATF/E4F binding site"
                     /note="Jones and Lee (1991), Mol Cell Biol 11:4297-4305
                     PMCID:PMC361290"
     promoter        4538..4546
                     /note="ATF binding site"
                     /note="Jones and Lee (1991), Mol Cell Biol 11:4297-4305
                     PMCID:PMC361290"
     regulatory      4562..4570
                     /regulatory_class="other"
                     /note="ATF/E4F binding site"
                     /note="Jones and Lee (1991), Mol Cell Biol 11:4297-4305
                     PMCID:PMC361290"
     promoter        4628..4636
                     /note="ATF binding site"
                     /note="Jones and Lee (1991), Mol Cell Biol 11:4297-4305
                     PMCID:PMC361290"
     repeat_region   complement(4629..4731)
                     /label=Ad5 right ITR
                     /note="Ad5 right ITR"
     promoter        4660..4668
                     /note="ATF binding site"
     promoter        4777..4881
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             4882..5742
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=M_Ampicillin-r_*
                     /note="Ampicillin-R"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5913..6501
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    6562..4324
                     /product="Scrambled Lambda DNA"
                     /label=Scrambled Lambda DNA
                     /note="Scrambled Lambda DNA"
ORIGIN
        1 tggccgttac gccaccagtc atcctcacga tgaaagtccg gcatctcaat ccccgccaga
       61 tgataagcat cccggaacag tgtgtaacag tccgtcaccg aaaactcctc cagtctgctg
      121 gcgttatcct ccgtcagctc gactttttcc agcagctcct tgccgagatg ggattcggtt
      181 atcttgcctt tgaaaaaatc caggtaacct tccgcatcat cgctcgcccg accgacggcc
      241 tccacgaatg ccgatttgcc aacggtgttc acactgcgga tataaaagta ataatcatgg
      301 cccggtttga tattgatact ggcggctatc cagtacagcg ccgtaccaag ataacgcgtg
      361 ctggtttcaa cctgtctgat atccgcaatc tgcttttccg agaaccagaa ctcaaactgt
      421 accgtcgggt cataaacggc aagatgcggc gtggcggtta tctgaaaata gcccggcgtc
      481 agctcaatcc tcgacggtgc tgccggtgcg gcaatccgga acgataccga cgccggatcg
      541 ccctgctgcc cccacgcatt taccgcccgg actgtcagcc tgtagttccc cagcgccagt
      601 tgcgtgaagc ggtatgtggt ttccgtcgtc cgggccgtgc tgaccagccg ctcactgccg
      661 tcgtccgctg ttacggtcag acggagcagg aaactcacgc ccttcaccac cttcggtgtg
      721 tcccatcgcg ccagcacctg atattccccg ctgtctgcag tgacttctgc ggtcaggtgc
      781 tgcaccgctg gcggcgtgac accattcacc gtgccactct gttcgccgtc aaagtgcgcc
      841 ccgttatcca cgatggcctc tttttccggc acatgctgca cggcggtgat ggcatacgtg
      901 ccgtcgtcgt tctcacggat actcacgcag cggaacagtc gctggcgcag cgtcggcagc
      961 ttcagctccc atacgctgta ttcagcaaca ccgtcaggaa cacggctcac ttttaccttc
     1021 acgccgtcgg tgacggactg aacctccacg ctgaccggat tgccacttcc gtcaaccagg
     1081 cttatcagcg cggtaccgga ggatggcagc gtgatttcac ggtcgagcgt cagcgtccgg
     1141 gtctggctgt tcaccgccag cacacgacca ccggtgctga taccggcata gtcatcatcg
     1201 cagatttcaa taacatcgcc cggtacatgg cgaagccctt ctgcgccgac gctgaaatcc
     1261 acggtctgcg tttccagcag ttctgtttta atcagccaca gcccggcgcg gtgtgcctgc
     1321 ccccggctgg tacagccaaa ggcatccatc ttcgtaacat tacgaccgta acgggcaatg
     1381 gcctgcgtat cttcaacaag ctctgtcgcc gtctcccagc cgttgttcgg gtcaatccag
     1441 ttcacctcaa cggcattatg gcggtccttc agggcgctga agctgtagcg gaacggcgcg
     1501 gaccgctata accgcactcg tcaccgcgat aggtccaggt gcaggtgttg gccagcatga
     1561 tacgtcccgg aaaaacagcg ccatccgttt ccgtcggcgt ggacagtaca aaggaggcac
     1621 tcaccgcgct cagttcgctg cactgctcaa tgcgccagcg gctgatcacc tcctgctccg
     1681 gatcggcgta actgtttccg ttgacgaagt tcaccgcatc cagaaaacgg gcgtaaacct
     1741 tacgccggac caccgttccg ccgaccagac tctgcatatc ttccgccatc ccggtgacca
     1801 taccgtacag gttagaaacc gtcagcgtgg ggcgcgtact ggtgcctttg ccattcagtt
     1861 caaaaccgct cccctgaatg ggatacggct gatactgtcg cccctgccag gtgaccggct
     1921 cacctttttc gttctgctca ttacagaaaa aataacgttc tccaccgacc tctggtcagg
     1981 tcgatttccc agagcaccac gctggccgac tgctccgcac gggtgcattc attcagtgtt
     2041 tcctgccgga tatcctgcat cagttcacca cctgttcaaa ctctgcgctg aactcaacac
     2101 gcagcatact gacccgcgac gaccattttg cgcaggtcac ctttatctgc cgccactcat
     2161 aaggcggcgt ccacagaaag gatttccagc ccccgtgctc ttccagaaac gactccagta
     2221 ccgtggcctc ctcacggggg acagaaagcg tcacgctgta cgttttcagg ttggcattca
     2281 gcccggcagg cgctcgctga gaatagccat caccaaagcg cacctttctt acagaaggga
     2341 ccgaagccac atccataccg ggtttcactt tccagcggaa ggtcttcatc gtccacctcc
     2401 ggagaacagg ccaccatcac gcatctgtgt ctgaatttca tcacgggcac ccttgcgggc
     2461 catgtcatac accgccttca gagcagccgg acctatctgc ccgttcgtgc cgtcgttgtt
     2521 aatcaccaca tggttattct gctcaaacgt cccggacgcc tgcgaccggc tgtctgccat
     2581 gctgcccggt gtaccgacat aaccgccggt ggcatagccg cgcatcagcc ggtaaagatt
     2641 ccccacgcca atccggctgg ttgcctcctt cgtgaagaca aactcaccac ggtgaacaat
     2701 ccccgctggc tcatatttgc cgccggttcc cgtaaatcct ccggttgcaa aatggaattt
     2761 cgccgcagcg gcctgaatgg ctgtaccgcc tgacgcggat gcgccgccac caacagcccc
     2821 gccaatggcg ctgccgatac tcccgacaat ccccaccatt gcctgcttaa gcagaatttc
     2881 tgtcatcatg gacagcacgg aacgggtgaa gctgcgccag ttctgctcac tgccggtcag
     2941 catcgccgcc atattctgtg caataccatc aaaggtctgc gtggctgcac tttttacctg
     3001 cgacatactg tccgtggcgc tctcttccca ctcactccag ccggacttca ggcctgccat
     3061 ccagttcccg cgaagctggt cttcagccgc ccaggtcttt ttctgctctg acatgacgtt
     3121 attcagcgcc agcggattat cgccatactg ttccttcagg cgctgttccg tggcttcccg
     3181 ttctgcctgc cggtcagtca gcccccggct tttcgcatca atggcggccc gttttgcccg
     3241 ttgctgctgt gcgaatttat ccgcctgctg cgccagcgcg ttcaggcgct cctgatacgt
     3301 aaccttgtcg ccaagtgcag ccagctggcg tttgtactcc agcgtctcat ctttatgcgc
     3361 cagcagggat ttctcctgtg cagacagctg gcgacgttgc gccgcctcct ccagtaccgc
     3421 gaactgactc tccgccttcc acaaatcccg gcgctgctgg ctgattttct catttgctcc
     3481 ggcatgcttc tccagcgtcc ggagttctgc ctgaagcgtc agcagggcag catgagcact
     3541 gtcttcctga cgatcgcccg cagacacctt cacgctggac tgtttcggct ttttcagcgt
     3601 cgcttcataa tcctttttcg ccgccgccat cagcgtgttg taatccgcct gcaggatttt
     3661 cccgtctttc agtgccttgt tcagttcttc ctgacgggcg gtatatttct ccagcggcgt
     3721 ctgcagccgt tcgtaagcct tctgcgcctc ttcggtatat ttcagccgtg acgcttcggt
     3781 atcgctctgc tgctgcgcat ttttgtcctg ttgagtctgc tgctcagcct tctttcgggc
     3841 ggcttcaagc gcaagacggg ccttttcacg atcatcccag taacgcgccc gcgcttcatc
     3901 gttaacaaaa taatcatcct tgcgcagatt ccagatgtcg tctgctttct tatacgcagc
     3961 ctctgcctta atcagcatct cctgcgcggt atcaggacga ccaatatcca gcaccgcatc
     4021 ccacatggat ttgaatgccc gcgcagtcct gtctgcccag gtctccagcg tgcccatgtt
     4081 ctctttcagg cggcgggtct ggtcatcaaa ccctttcgtt gcggcctcgt tcgccgcctg
     4141 caatgccccg gcttcatcgc cggaacgctg caactgagca acatacgcaa tctgctccgc
     4201 cgacacgtta tggaactggc gagccatcgc cgtcagcccc gacgtcgggt ctgtggtcag
     4261 cttcccgaag gcttcagcga cctttgtcca cctccacgcc ggatgcagag gagaaacgcg
     4321 ccacgatctt ctagaggcct agaaggccac atgacaagga ctatgtacta cgagtccaat
     4381 acactcatgg aggacggcca ccatggcctt atgtttcaac gtgtttattt ttcaattcta
     4441 aaaaatgacg taacggttaa agtccacaaa aaacacccag aaaaccgcac gcgaacctac
     4501 gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc cgttttccca
     4561 cgttacgtca cttcccattt taagaaaact acaattccca acacatacaa gttactccgc
     4621 cctaaaacct acgtcacccg ccccgttccc acgccccgcg ccacgtcaca aactccaccc
     4681 cctcattatc atattggctt caatccaaaa taaggtatat tattgatgat gttaattaat
     4741 ttaaatatcg caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4801 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4861 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4921 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4981 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5041 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5101 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5161 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5221 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5281 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5341 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5401 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5461 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5521 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5581 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5641 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5701 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5761 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5821 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5881 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5941 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6001 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6061 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6121 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6181 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6241 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6301 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6361 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6421 tatagtcctg ttgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6481 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6541 tgctggcctt ttgctcacat gtacggggag ttctggcttt cggtgccagc atctgcgcca
     6601 caccaccgag caccatactg gcaccgagag aaaacaggat gccggtcata ccaccggccc
     6661 caatggctgc cccccatgct gcaagggtgg ctccggcggt aaagaatgat ccggcaatgg
     6721 cggcagcccc caggacaatc tggaatacgc cacctgactt ggccccggcg actctgggaa
     6781 caatatgaat tacagcgcca tcaggcagag tctcatgtaa ctgcgccgtt aacccggacg
     6841 tgctgacgtc ccgcccggca atccgtacct gataccagcc gtcgctcagt ttctgacgaa
     6901 acgccgggag ctgtgtggcc agtgcccgga tggcttcagc ccccgttttc acacgaaggt
     6961 cgatgcggcg accaaatcgt tgtaaatccc cgtaaaggca gatgcgcgcg cgccatgccc
     7021 ggtgacgcca gagggagtgt gtgcgtcgct gccatttgtc ggtgtacctc tctcgtttgc
     7081 tcagttgttc aggaatatgg tgcagcagct cgccgtcgcc gcagtaaatt gcggcgtgat
     7141 tcggcactga tgaaccaaaa cagcacagca gcacatcgcc cggctgtgcc gctgacaacg
     7201 gcacctgata cagccccgtc gcctccagat tatccagata gagattc
//
