LOCUS Exported 7247 bp ds-DNA circular SYN 14-FEB-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAd1130-03 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7247) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Feb 14, 2017 from SnapGene 3.3.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7247 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 4446..4454 /regulatory_class="other" /note="ATF/E4F binding site" /note="Jones and Lee (1991), Mol Cell Biol 11:4297-4305 PMCID:PMC361290" promoter 4538..4546 /note="ATF binding site" /note="Jones and Lee (1991), Mol Cell Biol 11:4297-4305 PMCID:PMC361290" regulatory 4562..4570 /regulatory_class="other" /note="ATF/E4F binding site" /note="Jones and Lee (1991), Mol Cell Biol 11:4297-4305 PMCID:PMC361290" promoter 4628..4636 /note="ATF binding site" /note="Jones and Lee (1991), Mol Cell Biol 11:4297-4305 PMCID:PMC361290" repeat_region complement(4629..4731) /label=Ad5 right ITR /note="Ad5 right ITR" promoter 4660..4668 /note="ATF binding site" promoter 4777..4881 /gene="bla" /note="AmpR promoter" CDS 4882..5742 /codon_start=1 /product="beta-lactamase" /label=M_Ampicillin-r_* /note="Ampicillin-R" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 5913..6501 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 6562..4324 /product="Scrambled Lambda DNA" /label=Scrambled Lambda DNA /note="Scrambled Lambda DNA" ORIGIN 1 tggccgttac gccaccagtc atcctcacga tgaaagtccg gcatctcaat ccccgccaga 61 tgataagcat cccggaacag tgtgtaacag tccgtcaccg aaaactcctc cagtctgctg 121 gcgttatcct ccgtcagctc gactttttcc agcagctcct tgccgagatg ggattcggtt 181 atcttgcctt tgaaaaaatc caggtaacct tccgcatcat cgctcgcccg accgacggcc 241 tccacgaatg ccgatttgcc aacggtgttc acactgcgga tataaaagta ataatcatgg 301 cccggtttga tattgatact ggcggctatc cagtacagcg ccgtaccaag ataacgcgtg 361 ctggtttcaa cctgtctgat atccgcaatc tgcttttccg agaaccagaa ctcaaactgt 421 accgtcgggt cataaacggc aagatgcggc gtggcggtta tctgaaaata gcccggcgtc 481 agctcaatcc tcgacggtgc tgccggtgcg gcaatccgga acgataccga cgccggatcg 541 ccctgctgcc cccacgcatt taccgcccgg actgtcagcc tgtagttccc cagcgccagt 601 tgcgtgaagc ggtatgtggt ttccgtcgtc cgggccgtgc tgaccagccg ctcactgccg 661 tcgtccgctg ttacggtcag acggagcagg aaactcacgc ccttcaccac cttcggtgtg 721 tcccatcgcg ccagcacctg atattccccg ctgtctgcag tgacttctgc ggtcaggtgc 781 tgcaccgctg gcggcgtgac accattcacc gtgccactct gttcgccgtc aaagtgcgcc 841 ccgttatcca cgatggcctc tttttccggc acatgctgca cggcggtgat ggcatacgtg 901 ccgtcgtcgt tctcacggat actcacgcag cggaacagtc gctggcgcag cgtcggcagc 961 ttcagctccc atacgctgta ttcagcaaca ccgtcaggaa cacggctcac ttttaccttc 1021 acgccgtcgg tgacggactg aacctccacg ctgaccggat tgccacttcc gtcaaccagg 1081 cttatcagcg cggtaccgga ggatggcagc gtgatttcac ggtcgagcgt cagcgtccgg 1141 gtctggctgt tcaccgccag cacacgacca ccggtgctga taccggcata gtcatcatcg 1201 cagatttcaa taacatcgcc cggtacatgg cgaagccctt ctgcgccgac gctgaaatcc 1261 acggtctgcg tttccagcag ttctgtttta atcagccaca gcccggcgcg gtgtgcctgc 1321 ccccggctgg tacagccaaa ggcatccatc ttcgtaacat tacgaccgta acgggcaatg 1381 gcctgcgtat cttcaacaag ctctgtcgcc gtctcccagc cgttgttcgg gtcaatccag 1441 ttcacctcaa cggcattatg gcggtccttc agggcgctga agctgtagcg gaacggcgcg 1501 gaccgctata accgcactcg tcaccgcgat aggtccaggt gcaggtgttg gccagcatga 1561 tacgtcccgg aaaaacagcg ccatccgttt ccgtcggcgt ggacagtaca aaggaggcac 1621 tcaccgcgct cagttcgctg cactgctcaa tgcgccagcg gctgatcacc tcctgctccg 1681 gatcggcgta actgtttccg ttgacgaagt tcaccgcatc cagaaaacgg gcgtaaacct 1741 tacgccggac caccgttccg ccgaccagac tctgcatatc ttccgccatc ccggtgacca 1801 taccgtacag gttagaaacc gtcagcgtgg ggcgcgtact ggtgcctttg ccattcagtt 1861 caaaaccgct cccctgaatg ggatacggct gatactgtcg cccctgccag gtgaccggct 1921 cacctttttc gttctgctca ttacagaaaa aataacgttc tccaccgacc tctggtcagg 1981 tcgatttccc agagcaccac gctggccgac tgctccgcac gggtgcattc attcagtgtt 2041 tcctgccgga tatcctgcat cagttcacca cctgttcaaa ctctgcgctg aactcaacac 2101 gcagcatact gacccgcgac gaccattttg cgcaggtcac ctttatctgc cgccactcat 2161 aaggcggcgt ccacagaaag gatttccagc ccccgtgctc ttccagaaac gactccagta 2221 ccgtggcctc ctcacggggg acagaaagcg tcacgctgta cgttttcagg ttggcattca 2281 gcccggcagg cgctcgctga gaatagccat caccaaagcg cacctttctt acagaaggga 2341 ccgaagccac atccataccg ggtttcactt tccagcggaa ggtcttcatc gtccacctcc 2401 ggagaacagg ccaccatcac gcatctgtgt ctgaatttca tcacgggcac ccttgcgggc 2461 catgtcatac accgccttca gagcagccgg acctatctgc ccgttcgtgc cgtcgttgtt 2521 aatcaccaca tggttattct gctcaaacgt cccggacgcc tgcgaccggc tgtctgccat 2581 gctgcccggt gtaccgacat aaccgccggt ggcatagccg cgcatcagcc ggtaaagatt 2641 ccccacgcca atccggctgg ttgcctcctt cgtgaagaca aactcaccac ggtgaacaat 2701 ccccgctggc tcatatttgc cgccggttcc cgtaaatcct ccggttgcaa aatggaattt 2761 cgccgcagcg gcctgaatgg ctgtaccgcc tgacgcggat gcgccgccac caacagcccc 2821 gccaatggcg ctgccgatac tcccgacaat ccccaccatt gcctgcttaa gcagaatttc 2881 tgtcatcatg gacagcacgg aacgggtgaa gctgcgccag ttctgctcac tgccggtcag 2941 catcgccgcc atattctgtg caataccatc aaaggtctgc gtggctgcac tttttacctg 3001 cgacatactg tccgtggcgc tctcttccca ctcactccag ccggacttca ggcctgccat 3061 ccagttcccg cgaagctggt cttcagccgc ccaggtcttt ttctgctctg acatgacgtt 3121 attcagcgcc agcggattat cgccatactg ttccttcagg cgctgttccg tggcttcccg 3181 ttctgcctgc cggtcagtca gcccccggct tttcgcatca atggcggccc gttttgcccg 3241 ttgctgctgt gcgaatttat ccgcctgctg cgccagcgcg ttcaggcgct cctgatacgt 3301 aaccttgtcg ccaagtgcag ccagctggcg tttgtactcc agcgtctcat ctttatgcgc 3361 cagcagggat ttctcctgtg cagacagctg gcgacgttgc gccgcctcct ccagtaccgc 3421 gaactgactc tccgccttcc acaaatcccg gcgctgctgg ctgattttct catttgctcc 3481 ggcatgcttc tccagcgtcc ggagttctgc ctgaagcgtc agcagggcag catgagcact 3541 gtcttcctga cgatcgcccg cagacacctt cacgctggac tgtttcggct ttttcagcgt 3601 cgcttcataa tcctttttcg ccgccgccat cagcgtgttg taatccgcct gcaggatttt 3661 cccgtctttc agtgccttgt tcagttcttc ctgacgggcg gtatatttct ccagcggcgt 3721 ctgcagccgt tcgtaagcct tctgcgcctc ttcggtatat ttcagccgtg acgcttcggt 3781 atcgctctgc tgctgcgcat ttttgtcctg ttgagtctgc tgctcagcct tctttcgggc 3841 ggcttcaagc gcaagacggg ccttttcacg atcatcccag taacgcgccc gcgcttcatc 3901 gttaacaaaa taatcatcct tgcgcagatt ccagatgtcg tctgctttct tatacgcagc 3961 ctctgcctta atcagcatct cctgcgcggt atcaggacga ccaatatcca gcaccgcatc 4021 ccacatggat ttgaatgccc gcgcagtcct gtctgcccag gtctccagcg tgcccatgtt 4081 ctctttcagg cggcgggtct ggtcatcaaa ccctttcgtt gcggcctcgt tcgccgcctg 4141 caatgccccg gcttcatcgc cggaacgctg caactgagca acatacgcaa tctgctccgc 4201 cgacacgtta tggaactggc gagccatcgc cgtcagcccc gacgtcgggt ctgtggtcag 4261 cttcccgaag gcttcagcga cctttgtcca cctccacgcc ggatgcagag gagaaacgcg 4321 ccacgatctt ctagaggcct agaaggccac atgacaagga ctatgtacta cgagtccaat 4381 acactcatgg aggacggcca ccatggcctt atgtttcaac gtgtttattt ttcaattcta 4441 aaaaatgacg taacggttaa agtccacaaa aaacacccag aaaaccgcac gcgaacctac 4501 gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc cgttttccca 4561 cgttacgtca cttcccattt taagaaaact acaattccca acacatacaa gttactccgc 4621 cctaaaacct acgtcacccg ccccgttccc acgccccgcg ccacgtcaca aactccaccc 4681 cctcattatc atattggctt caatccaaaa taaggtatat tattgatgat gttaattaat 4741 ttaaatatcg caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 4801 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 4861 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 4921 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 4981 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 5041 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 5101 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 5161 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 5221 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 5281 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 5341 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 5401 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 5461 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 5521 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 5581 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 5641 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 5701 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 5761 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 5821 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 5881 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 5941 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 6001 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 6061 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 6121 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 6181 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 6241 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 6301 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 6361 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 6421 tatagtcctg ttgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 6481 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 6541 tgctggcctt ttgctcacat gtacggggag ttctggcttt cggtgccagc atctgcgcca 6601 caccaccgag caccatactg gcaccgagag aaaacaggat gccggtcata ccaccggccc 6661 caatggctgc cccccatgct gcaagggtgg ctccggcggt aaagaatgat ccggcaatgg 6721 cggcagcccc caggacaatc tggaatacgc cacctgactt ggccccggcg actctgggaa 6781 caatatgaat tacagcgcca tcaggcagag tctcatgtaa ctgcgccgtt aacccggacg 6841 tgctgacgtc ccgcccggca atccgtacct gataccagcc gtcgctcagt ttctgacgaa 6901 acgccgggag ctgtgtggcc agtgcccgga tggcttcagc ccccgttttc acacgaaggt 6961 cgatgcggcg accaaatcgt tgtaaatccc cgtaaaggca gatgcgcgcg cgccatgccc 7021 ggtgacgcca gagggagtgt gtgcgtcgct gccatttgtc ggtgtacctc tctcgtttgc 7081 tcagttgttc aggaatatgg tgcagcagct cgccgtcgcc gcagtaaatt gcggcgtgat 7141 tcggcactga tgaaccaaaa cagcacagca gcacatcgcc cggctgtgcc gctgacaacg 7201 gcacctgata cagccccgtc gcctccagat tatccagata gagattc //