LOCUS       pAd1129-34	5607 bp ds-DNA     circular SYN 12-DEC-2019
DEFINITION  pAd1129 derivative with 1222 bp BglII-MfeI deletion in E3 region and
            Ad5/3 fiber.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   OD260 INC
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 12, 2019 from SnapGene 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             9..284
                     /codon_start=1
                     /product="Ad5 E3 12.5K"
                     /label=Ad5 E3 12.5K
                     /db_xref="GI:56160551"
                     /protein_id="AP_000218.1"
                     /translation="MLSGEAEQLRLKHLVHCRRHKCFARDSGEFCYFELPEDHIEGPAH
                     GVRLTAQGELARSLIREFTQRPLLVERDRGPCVLTVICNCPNPGLHQ"
     CDS             449..730
                     /codon_start=1
                     /product="Ad5 E3 ADP 10.5 kD "
                     /label=Ad5 E3 ADP
                     /db_xref="GI:146161101"
                     /protein_id="Ap_000221.2"
                     /translation="MTNTTNAAAATGLTSTTNTPQVSAFVNNWDNLGMWWFSIALMFVC
                     LIIMWLICCLKRKRARPPIYSPIIVLHPNNDGIHRLDGLKHMFFSLTV"
     CDS             742..1017
                     /codon_start=1
                     /product="Ad5 E3 RID-alpha"
                     /label=Ad5 E3 RID-alpha
                     /db_xref="GI:56160556"
                     /protein_id="AP_000222.1"
                     /translation="MIPRVFILLTLVALFCACSTLAAVSHIEVDCIPAFTVYLLYGFVT
                     LTLICSLITVVIAFIQCIDWVCVRFAYLRHHPQYRDRTIAELLRIL"
     CDS             1020..1418
                     /codon_start=1
                     /product="Ad5 E3 RID-beta"
                     /label=Ad5 E3 RID-beta
                     /db_xref="GI:56160556"
                     /protein_id="AP_000223.1"
                     /translation="MKFTVTFLLIICTLSAFCSPTSKPQRHISCRFTRIWNIPSCYNEK
                     SDLSEAWLYAIISVMVFCSTILALAIYPYLDIGWNAIDAMNHPTFPAPAMLPLQQVVAG
                     GFVPANQPRPPSPTPTEISYFNLTGGDD"
     CDS             1411..1797
                     /codon_start=1
                     /product="Ad5 E3 14.7K"
                     /label=Ad5 E3 14.7K
                     /db_xref="GI:56160557"
                     /protein_id="AP_000224.1"
                     /translation="MTDTLDLEMDGIITEQRLLERRRAAAEQQRMNQELQDMVNLHQCK
                     RGIFCLVKQAKVTYDSNTTGHRLSYKLPTKRQKLVVMVGEKPITITQHSVETEGCIHSP
                     CQGPEDLCTLIKTLCGLKDLIPFN"
     CDS             complement(1826..1989)
                     /codon_start=1
                     /product="Ad5 E3 U Exon"
                     /label=Ad5 E3 U Exon
                     /db_xref="GI:56160558"
                     /protein_id="AP_000225.1"
                     /translation="MKIVGADGQEQEETDIPFRLWRKFAARRKLQYQSWEEGKEVLLNK
                     LDRNLLTDF"
     CDS             2000..3763
                     /codon_start=1
                     /product="Ad5/3 hybrid fiber"
                     /label=Ad5/3 Fiber
                     /db_xref="GI:56160559"
                     /protein_id="AP_000226.1"
                     /translation="MKRARPSEDTFNPVYPYDTETGPPTVPFLTPPFVSPNGFQESPPG
                     VLSLRLSEPLVTSNGMLALKMGNGLSLDEAGNLTSQNVTTVSPPLKKTKSNINLEISAP
                     LTVTSEALTVAAAAPLMVAGNTLTMQSQAPLTVHDSKLSIATQGPLTVSEGKLALQTSG
                     PLTTTDSSTLTITASPPLTTATGSLGIDLKEPIYTQNGKLGLKYGAPLHVTDDLNTLTV
                     ATGPGVTINNTSLQTKVTGALGFDSQGNMQLNVAGGLRIDSQNRRLILDVSYPFDAQNQ
                     LNLRLGQGPLFINSAHNLDINYNKGLYLFTASNNSKKLEVNLSTAKGLMFDATAIAINA
                     GDGLEFGSPNAPNTNPLKTKIGHGLEFDSNKAMVPKLGTGLSFDSTGAITVGNKNNDKL
                     TLWTGPKPEANCIIEYGKQNPDSKLTLILVKNGGIVNGYVTLMGASDYVNTLFKNKNVS
                     INVELYFDATGHILPDSSSLKTDLELKYKQTADFSARGFMPSTTAYPFVLPNAGTHNEN
                     YIFGQCYYKASDGALFPLEVTVMLNKRLPDSRTSYVMTFLWSLNAGLAPETTQATLITS
                     PFTFSYIREDD"
     promoter        join(2020,2106..2110)
                     /label=U exon Promoter
     regulatory      join(3762..3767,3777)
                     /regulatory_class="polyA_signal_sequence"
     rep_origin      complement(3848..4436)
                     /direction=LEFT
                     /label=pUC19 Ori
     CDS             complement(4607..5467)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=Ampicillin-R
                     /label=M_Ampicillin-r_*
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgaggccat gttaagtgga gaggcagagc aactgcgcct gaaacacctg gtccactgtc
       61 gccgccacaa gtgctttgcc cgcgactccg gtgagttttg ctactttgaa ttgcccgagg
      121 atcatatcga gggcccggcg cacggcgtcc ggcttaccgc ccagggagag cttgcccgta
      181 gcctgattcg ggagtttacc cagcgccccc tgctagttga gcgggacagg ggaccctgtg
      241 ttctcactgt gatttgcaac tgtcctaacc ctggattaca tcaagatctc ctaggctagc
      301 actagttcta gacaattgac tctatgtggg atatgctcca gcgctacaac cttgaagtca
      361 ggcttcctgg atgtcagcat ctgactttgg ccagcacctg tcccgcggat ttgttccagt
      421 ccaactacag cgacccaccc taacagagat gaccaacaca accaacgcgg ccgccgctac
      481 cggacttaca tctaccacaa atacacccca agtttctgcc tttgtcaata actgggataa
      541 cttgggcatg tggtggttct ccatagcgct tatgtttgta tgccttatta ttatgtggct
      601 catctgctgc ctaaagcgca aacgcgcccg accacccatc tatagtccca tcattgtgct
      661 acacccaaac aatgatggaa tccatagatt ggacggactg aaacacatgt tcttttctct
      721 tacagtatga ttaaatgaga catgattcct cgagttttta tattactgac ccttgttgcg
      781 cttttttgtg cgtgctccac attggctgcg gtttctcaca tcgaagtaga ctgcattcca
      841 gccttcacag tctatttgct ttacggattt gtcaccctca cgctcatctg cagcctcatc
      901 actgtggtca tcgcctttat ccagtgcatt gactgggtct gtgtgcgctt tgcatatctc
      961 agacaccatc cccagtacag ggacaggact atagctgagc ttcttagaat tctttaatta
     1021 tgaaatttac tgtgactttt ctgctgatta tttgcaccct atctgcgttt tgttccccga
     1081 cctccaagcc tcaaagacat atatcatgca gattcactcg tatatggaat attccaagtt
     1141 gctacaatga aaaaagcgat ctttccgaag cctggttata tgcaatcatc tctgttatgg
     1201 tgttctgcag taccatctta gccctagcta tatatcccta ccttgacatt ggctggaacg
     1261 caatagatgc catgaaccac ccaactttcc ccgcgcccgc tatgcttcca ctgcaacaag
     1321 ttgttgccgg cggctttgtc ccagccaatc agcctcgccc accttctccc acccccactg
     1381 aaatcagcta ctttaatcta acaggaggag atgactgaca ccctagatct agaaatggac
     1441 ggaattatta cagagcagcg cctgctagaa agacgcaggg cagcggccga gcaacagcgc
     1501 atgaatcaag agctccaaga catggttaac ttgcaccagt gcaaaagggg tatcttttgt
     1561 ctggtaaagc aggccaaagt cacctacgac agtaatacca ccggacaccg ccttagctac
     1621 aagttgccaa ccaagcgtca gaaattggtg gtcatggtgg gagaaaagcc cattaccata
     1681 actcagcact cggtagaaac cgaaggctgc attcactcac cttgtcaagg acctgaggat
     1741 ctctgcaccc ttattaagac cctgtgcggt ctcaaagatc ttattccctt taactaataa
     1801 aaaaaaataa taaagcatca cttacttaaa atcagttagc aaatttctgt ccagtttatt
     1861 cagcagcacc tccttgccct cctcccagct ctggtattgc agcttcctcc tggctgcaaa
     1921 ctttctccac aatctaaatg gaatgtcagt ttcctcctgt tcctgtccat ccgcacccac
     1981 tatcttcatg ttgttgcaga tgaagcgcgc aagaccgtct gaagatacct tcaaccccgt
     2041 gtatccatat gacacggaaa ccggtcctcc aactgtgcct tttcttactc ctccctttgt
     2101 atcccccaat gggtttcaag agagtccccc tggggtactc tctttgcgcc tatccgaacc
     2161 tctagttacc tccaatggca tgcttgcgct caaaatgggc aacggcctct ctctggacga
     2221 ggccggcaac cttacctccc aaaatgtaac cactgtgagc ccacctctca aaaaaaccaa
     2281 gtcaaacata aacctggaaa tatctgcacc cctcacagtt acctcagaag ccctaactgt
     2341 ggctgccgcc gcacctctaa tggtcgcggg caacacactc accatgcaat cacaggcccc
     2401 gctaaccgtg cacgactcca aacttagcat tgccacccaa ggacccctca cagtgtcaga
     2461 aggaaagcta gccctgcaaa catcaggccc cctcaccacc accgatagca gtacccttac
     2521 tatcactgcc tcaccccctc taactactgc cactggtagc ttgggcattg acttgaaaga
     2581 gcccatttat acacaaaatg gaaaactagg actaaagtac ggggctcctt tgcatgtaac
     2641 agacgaccta aacactttga ccgtagcaac tggtccaggt gtgactatta ataatacttc
     2701 cttgcaaact aaagttactg gagccttggg ttttgattca caaggcaata tgcaacttaa
     2761 tgtagcagga ggactaagga ttgattctca aaacagacgc cttatacttg atgttagtta
     2821 tccgtttgat gctcaaaacc aactaaatct aagactagga cagggccctc tttttataaa
     2881 ctcagcccac aacttggata ttaactacaa caaaggcctt tacttgttta cagcttcaaa
     2941 caattccaaa aagcttgagg ttaacctaag cactgccaag gggttgatgt ttgacgctac
     3001 agccatagcc attaatgcag gagatgggct tgaatttggt tcacctaatg caccaaacac
     3061 aaatcccctc aaaacaaaaa ttggccatgg cctagaattt gattcaaaca aggctatggt
     3121 tcctaaacta ggaactggcc ttagttttga cagcacaggt gccattacag taggaaacaa
     3181 aaataatgat aagctaaccc tatggacagg tccaaaacca gaagccaact gcataattga
     3241 atacgggaaa caaaacccag atagcaaact aactttaatc cttgtaaaaa atggaggaat
     3301 tgttaatgga tatgtaacgc taatgggagc ctcagactac gttaacacct tatttaaaaa
     3361 caaaaatgtc tccattaatg tagaactata ctttgatgcc actggtcata tattaccaga
     3421 ctcatcttct cttaaaacag atctagaact aaaatacaag caaaccgctg actttagtgc
     3481 aagaggtttt atgccaagta ctacagcgta tccatttgtc cttcctaatg cgggaacaca
     3541 taatgaaaat tatatttttg gtcaatgcta ctacaaagca agcgatggtg ccctttttcc
     3601 gttggaagtt actgttatgc ttaataaacg cctgccagat agtcgcacat cctatgttat
     3661 gactttttta tggtccttga atgctggtct agctccagaa actactcagg caaccctcat
     3721 aacctcccca tttacctttt cctatattag agaagatgac taataaagaa tcgtttgtgg
     3781 gccaccatgg ccgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4261 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4921 ctacaggcat cgtggtgtcg cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5221 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacattcc
     5581 ccgaaaagtg ccacctgacg tcggcca
//
