LOCUS       pAd1129-06                3413 bp ds-DNA     circular SYN 23-FEB-2018
DEFINITION  pAd1129-06 is a shuttle plasmid designed for constructing E3-deleted
            and bipartite (dicistronic) recombinant adenovirus vectors that are 
            targeted to cells expressing the CD46 receptor.  It contains the 
            sequence encompassing psn 27885-32795 in the Ad5 genome including 
            the E3 region and a Ad5/35 hybrid fiber gene.  Almost all E3 genes 
            (2.7 kb BglII fragment including gp19K membrane protein and the 
            adenovirus "death" protein) were deleted, and replaced with a 
            multiple cloning site, which allows for the insertion of expression 
            cassettes in the E3 region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   OD260 Inc
  TITLE     Direct Submission
  JOURNAL   Exported Feb 23, 2018 from SnapGene 4.1.5
            http://www.snapgene.com
COMMENT     Alias: pAd1129-06R2
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             6..285
                     /codon_start=1
                     /product="Ad5 E3 12.5K"
                     /label=Ad5 E3 12.5K (truncated)
                     /label=Ad5 E3 12.5K
                     /db_xref="GI:56160551"
                     /protein_id="AP_000218.1"
                     /translation="MLSGEAEQLRLKHLVHCRRHKCFARDSGEFCYFELPEDHIEGPAH
                     GVRLTAQGELARSLIREFTQRPLLVERDRGPCVLTVICNCPNPGLHQD"
     misc_feature    287..343
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(514..568)
                     /codon_start=1
                     /product="Ad5 E3 U Exon"
                     /label=Ad5 E3 U Exon
                     /db_xref="GI:56160558"
                     /protein_id="AP_000225.1"
                     /translation="MKIVGADGQEQEETDIPF"
     CDS             579..584
                     /codon_start=1
                     /product="Ad5 protein IV, which trimerizes to form the 
                     fiber.  The fiber and protein III form the penton base 
                     located at the vertices of the icosahedron."
                     /label=Ad5 Fiber
                     /db_xref="GI:56160559"
                     /protein_id="AP_000226.1"
                     /translation="MK"
     CDS             585..1546
                     /codon_start=1
                     /label=Ad5/35 Fiber
                     /translation="RARPSEDTFNPVYPYDTETGPPTVPFLTPPFVSPNGFQESPPGVL
                     TLKCLTPLTTTGGSLQLKVGGGLTVDDTDGTLQENIRATAPITKNNHSVELSIGNGLET
                     QNNKLCAKLGNGLKFNNGDICIKDSINTLWTGINPPPNCQIVENTNTNDGKLTLVLVKN
                     GGLVNGYVSLVGVSDTVNQMFTQKTANIQLRLYFDSSGNLLTDESDLKIPLKNKSSTAT
                     SETVASSKAFMPSTTAYPFNTTTRDSENYIHGICYYMTSYDRSLFPLNISIMLNSRMIS
                     SNVAYAIQFEWNLNASESPESNIATLTTSPFFFSYITED"
     regulatory      join(1564..1569,1579)
                     /regulatory_class="polyA_signal_sequence"
                     /note="Ad5 L5 polyA signal
                     (Le Moullec et al, 1983, J. Virol. 48:127-134)"
     rep_origin      complement(1650..2238)
                     /direction=LEFT
                     /label=pUC19 Ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2409..3269)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=Ampicillin-R
                     /label=M_Ampicillin-r_*
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aggccatgtt aagtggagag gcagagcaac tgcgcctgaa acacctggtc cactgtcgcc
       61 gccacaagtg ctttgcccgc gactccggtg agttttgcta ctttgaattg cccgaggatc
      121 atatcgaggg cccggcgcac ggcgtccggc ttaccgccca gggagagctt gcccgtagcc
      181 tgattcggga gtttacccag cgccccctgc tagttgagcg ggacagggga ccctgtgttc
      241 tcactgtgat ttgcaactgt cctaaccctg gattacatca agatccagat cttctagacc
      301 cgggagcggc cgctgtcgac ctgcaggatc cgaattcgat atcactagtg gtaccgatct
      361 tattcccttt aactaataaa aaaaaataat aaagcatcac ttacttaaaa tcagttagca
      421 aatttctgtc cagtttattc agcagcacct ccttgccctc ctcccagctc tggtattgca
      481 gcttcctcct ggctgcaaac tttctccaca atctaaatgg aatgtcagtt tcctcctgtt
      541 cctgtccatc cgcacccact atcttcatgt tgttgcagat gaagcgcgca agaccgtctg
      601 aagatacctt caaccccgtg tatccatatg acactgaaac cggtcctcca actgtgcctt
      661 ttcttactcc tccctttgta tcccccaatg ggtttcaaga gagtccccct ggagttctta
      721 ctttaaaatg tttaacccca ctaacaacca caggcggatc tctacagcta aaagtgggag
      781 ggggacttac agtggatgac actgatggta ccttacaaga aaacatacgt gctacagcac
      841 ccattactaa aaataatcac tctgtagaac tatccattgg aaatggatta gaaactcaaa
      901 acaataaact atgtgccaaa ttgggaaatg ggttaaaatt taacaacggt gacatttgta
      961 taaaggatag tattaacacc ttatggactg gaataaaccc tccacctaac tgtcaaattg
     1021 tggaaaacac taatacaaat gatggcaaac ttactttagt attagtaaaa aacggagggc
     1081 ttgttaatgg ctacgtgtct ctagttggtg tatcagacac tgtgaaccaa atgttcacac
     1141 aaaagacagc aaacatccaa ttaagattat attttgactc ttctggaaat ctattaactg
     1201 atgaatcaga cttaaaaatt ccacttaaaa ataaatcttc tacagcgacc agtgaaactg
     1261 tagccagcag caaagccttt atgccaagta ctacagctta tcccttcaac accactacta
     1321 gggatagtga aaactacatt catggaatat gttactacat gactagttat gatagaagtc
     1381 tatttccctt gaacatttct ataatgctaa acagccgtat gatttcttcc aatgttgcct
     1441 atgccataca atttgaatgg aatctaaatg caagtgaatc tccagaaagc aacatagcta
     1501 cgctgaccac atcccccttt ttcttttctt acattacaga agacgacaac taaaatgccc
     1561 aagaataaag aatcgtttgt gggccaccat ggccgagcaa aaggccagca aaaggccagg
     1621 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1681 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1741 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1801 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1861 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1921 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1981 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2041 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2101 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2161 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2221 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2281 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2341 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2401 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2461 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2521 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2581 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2641 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2701 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2761 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2821 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2881 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2941 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3001 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3061 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3121 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3181 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3241 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3301 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3361 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcggcca atg
//
