LOCUS       pAd1129-02               4168 bp DNA     circular
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   OD260 Inc
  TITLE     Direct Submission
  JOURNAL   .
COMMENT     .
FEATURES             Location/Qualifiers
     source          1..4168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9..288
                     /codon_start=1
                     /product="Ad5 E3 12.5K"
                     /label=Ad5 E3 12.5K (truncated)
                     /label=Ad5 E3 12.5K
                     /db_xref="GI:56160551"
                     /protein_id="AP_000218.1"
                     /translation="MLSGEAEQLRLKHLVHCRRHKCFARDSGEFCYFELPEDHIEGPAH
                     GVRLTAQGELARSLIREFTQRPLLVERDRGPCVLTVICNCPNPGLHQD"
     misc_feature    290..358
                     /label=MCS-1
                     /note="Multiple cloning site"
     CDS             complement(408..571)
                     /codon_start=1
                     /product="Ad5 E3 U Exon"
                     /label=Ad5 E3 U Exon
                     /db_xref="GI:56160558"
                     /protein_id="AP_000225.1"
                     /translation="MKIVGADGQEQEETDIPFRLWRKFAARRKLQYQSWEEGKEVLLNK
                     LDRNLLTDF"
     CDS             582..2327
                     /codon_start=1
                     /product="Ad5 protein IV, which trimerizes to form the 
                     fiber.  The fiber and protein III form the penton base 
                     located at the vertices of the icosahedron."
                     /label=Ad5 Fiber
                     /db_xref="GI:56160559"
                     /protein_id="AP_000226.1"
                     /translation="MKRARPSEDTFNPVYPYDTETGPPTVPFLTPPFVSPNGFQESPPG
                     VLSLRLSEPLVTSNGMLALKMGNGLSLDEAGNLTSQNVTTVSPPLKKTKSNINLEISAP
                     LTVTSEALTVAAAAPLMVAGNTLTMQSQAPLTVHDSKLSIATQGPLTVSEGKLALQTSG
                     PLTTTDSSTLTITASPPLTTATGSLGIDLKEPIYTQNGKLGLKYGAPLHVTDDLNTLTV
                     ATGPGVTINNTSLQTKVTGALGFDSQGNMQLNVAGGLRIDSQNRRLILDVSYPFDAQNQ
                     LNLRLGQGPLFINSAHNLDINYNKGLYLFTASNNSKKLEVNLSTAKGLMFDATAIAINA
                     GDGLEFGSPNAPNTNPLKTKIGHGLEFDSNKAMVPKLGTGLSFDSTGAITVGNKNNDKL
                     TLWTTPAPSPNCRLNAEKDAKLTLVLTKCGSQILATVSVLAVKGSLAPISGTVQSAHLI
                     IRFDENGVLLNNSFLDPEYWNFRNGDLTEGTAYTNAVGFMPNLSAYPKSHGKTAKSNIV
                     SQVYLNGDKTKPVTLTITLNGTQETGDTTPSAYSMSFSWDWSGHNYINEIFATSSYTFS
                     YIAQE"
     regulatory      join(2323..2328,2338)
                     /label=Ad5 L5 polyA
                     /note="Ad5 L5 polyA signal(Le Moullec et al, 1983, J. 
                     Virol. 48:127-134)
                     "
                     /regulatory_class="polyA_signal_sequence"
     rep_origin      complement(2409..2997)
                     /direction=LEFT
                     /label=pUC19 Ori
     CDS             complement(3168..4028)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=Ampicillin-R
                     /label=M_Ampicillin-r_*
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgaggccat gttaagtgga gaggcagagc aactgcgcct gaaacacctg gtccactgtc
       61 gccgccacaa gtgctttgcc cgcgactccg gtgagttttg ctactttgaa ttgcccgagg
      121 atcatatcga gggcccggcg cacggcgtcc ggcttaccgc ccagggagag cttgcccgta
      181 gcctgattcg ggagtttacc cagcgccccc tgctagttga gcgggacagg ggaccctgtg
      241 ttctcactgt gatttgcaac tgtcctaacc ctggattaca tcaagatcca gatcttctag
      301 acccgggagc ggccgctgtc gacctgcagg atccgaattc gatatcacta gtggtaccga
      361 tcttattccc tttaactaat aaaaaaaaat aataaagcat cacttactta aaatcagtta
      421 gcaaatttct gtccagttta ttcagcagca cctccttgcc ctcctcccag ctctggtatt
      481 gcagcttcct cctggctgca aactttctcc acaatctaaa tggaatgtca gtttcctcct
      541 gttcctgtcc atccgcaccc actatcttca tgttgttgca gatgaagcgc gcaagaccgt
      601 ctgaagatac cttcaacccc gtgtatccat atgacacgga aaccggtcct ccaactgtgc
      661 cttttcttac tcctcccttt gtatccccca atgggtttca agagagtccc cctggggtac
      721 tctctttgcg cctatccgaa cctctagtta cctccaatgg catgcttgcg ctcaaaatgg
      781 gcaacggcct ctctctggac gaggccggca accttacctc ccaaaatgta accactgtga
      841 gcccacctct caaaaaaacc aagtcaaaca taaacctgga aatatctgca cccctcacag
      901 ttacctcaga agccctaact gtggctgccg ccgcacctct aatggtcgcg ggcaacacac
      961 tcaccatgca atcacaggcc ccgctaaccg tgcacgactc caaacttagc attgccaccc
     1021 aaggacccct cacagtgtca gaaggaaagc tagccctgca aacatcaggc cccctcacca
     1081 ccaccgatag cagtaccctt actatcactg cctcaccccc tctaactact gccactggta
     1141 gcttgggcat tgacttgaaa gagcccattt atacacaaaa tggaaaacta ggactaaagt
     1201 acggggctcc tttgcatgta acagacgacc taaacacttt gaccgtagca actggtccag
     1261 gtgtgactat taataatact tccttgcaaa ctaaagttac tggagccttg ggttttgatt
     1321 cacaaggcaa tatgcaactt aatgtagcag gaggactaag gattgattct caaaacagac
     1381 gccttatact tgatgttagt tatccgtttg atgctcaaaa ccaactaaat ctaagactag
     1441 gacagggccc tctttttata aactcagccc acaacttgga tattaactac aacaaaggcc
     1501 tttacttgtt tacagcttca aacaattcca aaaagcttga ggttaaccta agcactgcca
     1561 aggggttgat gtttgacgct acagccatag ccattaatgc aggagatggg cttgaatttg
     1621 gttcacctaa tgcaccaaac acaaatcccc tcaaaacaaa aattggccat ggcctagaat
     1681 ttgattcaaa caaggctatg gttcctaaac taggaactgg ccttagtttt gacagcacag
     1741 gtgccattac agtaggaaac aaaaataatg ataagctaac tttgtggacc acaccagctc
     1801 catctcctaa ctgtagacta aatgcagaga aagatgctaa actcactttg gtcttaacaa
     1861 aatgtggcag tcaaatactt gctacagttt cagttttggc tgttaaaggc agtttggctc
     1921 caatatctgg aacagttcaa agtgctcatc ttattataag atttgacgaa aatggagtgc
     1981 tactaaacaa ttccttcctg gacccagaat attggaactt tagaaatgga gatcttactg
     2041 aaggcacagc ctatacaaac gctgttggat ttatgcctaa cctatcagct tatccaaaat
     2101 ctcacggtaa aactgccaaa agtaacattg tcagtcaagt ttacttaaac ggagacaaaa
     2161 ctaaacctgt aacactaacc attacactaa acggtacaca ggaaacagga gacacaactc
     2221 caagtgcata ctctatgtca ttttcatggg actggtctgg ccacaactac attaatgaaa
     2281 tatttgccac atcctcttac actttttcat acattgccca agaataaaga atcgtttgtg
     2341 ggccaccatg gccgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2401 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2461 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2521 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2581 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2641 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2701 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2761 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2821 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2881 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2941 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3001 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3061 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3121 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3181 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3241 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3301 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3361 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3421 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3481 gctacaggca tcgtggtgtc gcgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3541 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3601 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3661 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3721 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3781 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3841 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3901 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3961 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4021 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4081 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattc
     4141 cccgaaaagt gccacctgac gtcggcca
//
