LOCUS       pAd1127-08                3986 bp ds-DNA     circular SYN 23-FEB-2018
DEFINITION  pAd1127-08 is a plasmid designed for constructing adenovirus vectors
            expressing transgenes under the control of a CMV promoter located in
            place of the E1 region of the Ad5 genome.   It is a derivative of 
            pAd1127-02, in which a cassette containing a CMV promoter- MCS- 
            bovine growth hormone (bGH) polyA signal was inserted between the 
            XbaI and Acc65I sites in counterclockwise orientation, i.e. towards 
            the left end of the adenovirus genome.  pAd1127-08 contains PacI and
            SwaI sites flanking the first 350 base pairs from the Ad5 genome 
            (including the left ITR and packaging signal).  The sequences 
            encompassing the kanamycin-resistance gene, the lambda cos site, the
            adenovirus 0-1 map units, the CMV expression cassette and the pIX 
            coding sequence are flanked by two SfiI restriction sites.  These 
            sites generate non-symmetrical sticky ends suitable for directional 
            cloning with the other AdenoQuick2.0 plasmids (pAd1128, pAd1129, 
            pAd1130, and their derivatives).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   OD260 Inc
  TITLE     Direct Submission
  JOURNAL   Exported Feb 23, 2018 from SnapGene 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   4..106
                     /label=Ad5 left ITR
                     /note="ITR"
     regulatory      28..40
                     /regulatory_class="enhancer"
     promoter        67..75
                     /label=ATF binding site
     misc_signal     194..344
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     misc_feature    243..251
                     /function="Ad5 genome packaging"
                     /label=A1
                     /note="- A1 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    248..261
                     /function="Ad5 genome packaging"
                     /label=A1
                     /note="- A1 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'"
     misc_feature    263..272
                     /function="Ad5 genome packaging"
                     /label=A2
                     /note="- A2 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    269..282
                     /function="Ad5 genome packaging"
                     /label=A2
                     /note="- A2 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'"
     misc_feature    305..314
                     /function="Ad5 DNA packaging"
                     /label=A3
                     /note="- A3 repeat
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    316..324
                     /function="Ad5 DNA packaging"
                     /label=A4
                     /note="- A4 repeat
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    340..349
                     /function="Ad5 DNA packaging"
                     /label=A5
                     /note="- A5 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     polyA_signal    390..614
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        640..658
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    662..761
                     /label=MCS
                     /note="Multiple cloning site"
     promoter        complement(775..793)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(838..1041)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1042..1421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             1527..1949
                     /codon_start=1
                     /gene="pIX"
                     /product="Ad5 pIX"
                     /label=Ad5 pIX
                     /db_xref="GI:33465835"
                     /protein_id="AAQ19288.1"
                     /translation="MSTNSFDGSIVSSYLTTRMPPWAGVRQNVMGSSIDGRPVLPANST
                     TLTYETVSGTPLETAASAAASAAAATARGIVTDFAFLSPLASSAASRSSARDDKLTALL
                     AQLDSLTRELNVVSQQLLDLRQQVSALKASSPPNAV"
     rep_origin      complement(2025..2613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2788..3603)
                     /codon_start=1
                     /product="Kanamycin-R"
                     /label=Kanamycin-R
                     /note="origin: Tn903"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_signal     3666..3913
                     /label=Cos site
                     /note="phage lambda COS site"
ORIGIN
        1 taacatcatc aataatatac cttattttgg attgaagcca atatgataat gagggggtgg
       61 agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg acgtagtagt gtggcggaag
      121 tgtgatgttg caagtgtggc ggaacacatg taagcgacgg atgtggcaaa agtgacgttt
      181 ttggtgtgcg ccggtgtaca caggaagtga caattttcgc gcggttttag gcggatgttg
      241 tagtaaattt gggcgtaacc gagtaagatt tggccatttt cgcgggaaaa ctgaataaga
      301 ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg taatatttgt ctagggagat
      361 cttctagctg gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct
      421 gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga
      481 atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg
      541 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
      601 actagaaggc acagtcgagg ctgatcagcg agctctagca tttaggtgac actatagaat
      661 agggccctct agatgcatgc tcgagcggcc gccagtgtga tggatatctg cagaattcca
      721 gcacactggc ggccgttact agtggatccg agctcggtac caagcttggg tctccctata
      781 gtgagtcgta ttaatttcga taagccagta agcagtgggt tctctagtta gccagagagc
      841 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
      901 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
      961 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
     1021 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
     1081 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     1141 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     1201 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     1261 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     1321 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
     1381 cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgagt actgaaatgt
     1441 gtgggcgtgg cttaagggtg ggaaagaata tataaggtgg gggtcttatg tagttttgta
     1501 tctgttttgc agcagccgcc gccgccatga gcaccaactc gtttgatgga agcattgtga
     1561 gctcatattt gacaacgcgc atgcccccat gggccggggt gcgtcagaat gtgatgggct
     1621 ccagcattga tggtcgcccc gtcctgcccg caaactctac taccttgacc tacgagaccg
     1681 tgtctggaac gccgttggag actgcagcct ccgccgccgc ttcagccgct gcagccaccg
     1741 cccgcgggat tgtgactgac tttgctttcc tgagcccgct tgcaagcagt gcagcttccc
     1801 gttcatccgc ccgcgatgac aagttgacgg ctcttttggc acaattggat tctttgaccc
     1861 gggaacttaa tgtcgtttct cagcagctgt tggatctgcg ccagcaggtt tctgccctga
     1921 aggcttcctc ccctcccaat gcggtttaac ggtccgggcc agagtggccg agcaaaaggc
     1981 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2041 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2101 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2161 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2221 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2281 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2341 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2401 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2461 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2521 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2581 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2641 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2701 aggatcttcg gccaagaagg ccagagtggc agagactgca ttcgaaaacg tttgaattga
     2761 taattattat catttgcggg tcaattctta gaaaaactca tcgagcatca aatgaaactg
     2821 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     2881 aggagaaaac tcaccgaggc agttccatag gattgcaaga tcctggtatc ggtctgcgat
     2941 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     3001 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat
     3061 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     3121 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
     3181 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     3241 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg
     3301 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     3361 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     3421 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
     3481 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     3541 agcatccatg ttggaattta atcgcggcct cgagcaagac gtttcccgtt gaatatggct
     3601 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgcgaaaac
     3661 gtttgaattg ataattatta tcatttgcgg gtcctttccg gcgatccgcc ttgttacggg
     3721 gcggcgacct cgcgggtttt cgctatttat gaaaattttc cggtttaagg cgtttccgtt
     3781 cttcttcgtc ataacttaat gtttttattt aaaataccct ctgaaaagaa aggaaacgac
     3841 agctgaaagc gagctttttg gcctctgtcg tttcctttct ctgtttttgt ccgtggaatg
     3901 aacaatggaa gtcggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     3961 ggtttcttag cgatatttaa attaat
//
