LOCUS       pAd1127-07		3987 bp ds-DNA     circular SYN 24-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   OD260 Inc
  TITLE     Direct Submission
  JOURNAL   
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   4..106
                     /label=Ad5 left ITR
                     /note="ITR"
     misc_signal     194..344
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        372..751
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        752..955
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    941..947
                     /label=TATA box
     promoter        1000..1018
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1135..1153)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1179..1403
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             1528..1950
                     /codon_start=1
                     /gene="pIX"
                     /product="Ad5 pIX"
                     /label=Ad5 pIX
                     /db_xref="GI:33465835"
                     /protein_id="AAQ19288.1"
                     /translation="MSTNSFDGSIVSSYLTTRMPPWAGVRQNVMGSSIDGRPVLPANST
                     TLTYETVSGTPLETAASAAASAAAATARGIVTDFAFLSPLASSAASRSSARDDKLTALL
                     AQLDSLTRELNVVSQQLLDLRQQVSALKASSPPNAV"
     rep_origin      complement(2026..2614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2789..3604)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_signal     3667..3914
                     /label=Cos site
                     /note="phage lambda COS site"
ORIGIN
        1 taacatcatc aataatatac cttattttgg attgaagcca atatgataat gagggggtgg
       61 agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg acgtagtagt gtggcggaag
      121 tgtgatgttg caagtgtggc ggaacacatg taagcgacgg atgtggcaaa agtgacgttt
      181 ttggtgtgcg ccggtgtaca caggaagtga caattttcgc gcggttttag gcggatgttg
      241 tagtaaattt gggcgtaacc gagtaagatt tggccatttt cgcgggaaaa ctgaataaga
      301 ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg taatatttgt ctagggagat
      361 cttctcgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      421 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      481 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      541 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      601 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      661 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      721 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      781 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      841 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      901 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
      961 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
     1021 gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt gctggaattc
     1081 tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc ctattctata
     1141 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     1201 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     1261 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     1321 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     1381 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagaccag tactgaaatg
     1441 tgtgggcgtg gcttaagggt gggaaagaat atataaggtg ggggtcttat gtagttttgt
     1501 atctgttttg cagcagccgc cgccgccatg agcaccaact cgtttgatgg aagcattgtg
     1561 agctcatatt tgacaacgcg catgccccca tgggccgggg tgcgtcagaa tgtgatgggc
     1621 tccagcattg atggtcgccc cgtcctgccc gcaaactcta ctaccttgac ctacgagacc
     1681 gtgtctggaa cgccgttgga gactgcagcc tccgccgccg cttcagccgc tgcagccacc
     1741 gcccgcggga ttgtgactga ctttgctttc ctgagcccgc ttgcaagcag tgcagcttcc
     1801 cgttcatccg cccgcgatga caagttgacg gctcttttgg cacaattgga ttctttgacc
     1861 cgggaactta atgtcgtttc tcagcagctg ttggatctgc gccagcaggt ttctgccctg
     1921 aaggcttcct cccctcccaa tgcggtttaa cggtccgggc cagagtggcc gagcaaaagg
     1981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2221 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2461 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2581 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2641 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2701 aaggatcttc ggccaagaag gccagagtgg cagagactgc attcgaaaac gtttgaattg
     2761 ataattatta tcatttgcgg gtcaattctt agaaaaactc atcgagcatc aaatgaaact
     2821 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     2881 aaggagaaaa ctcaccgagg cagttccata ggattgcaag atcctggtat cggtctgcga
     2941 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     3001 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca
     3061 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     3121 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     3181 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     3241 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     3301 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     3361 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     3421 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     3481 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     3541 cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc
     3601 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgcgaaaa
     3661 cgtttgaatt gataattatt atcatttgcg ggtcctttcc ggcgatccgc cttgttacgg
     3721 ggcggcgacc tcgcgggttt tcgctattta tgaaaatttt ccggtttaag gcgtttccgt
     3781 tcttcttcgt cataacttaa tgtttttatt taaaataccc tctgaaaaga aaggaaacga
     3841 cagctgaaag cgagcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat
     3901 gaacaatgga agtcggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     3961 tggtttctta gcgatattta aattaat
//
