LOCUS       Exported                3071 bp ds-DNA     circular SYN 16-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAd1127-06
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Xavier Danthinne
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 16, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     repeat_region   4..106
                     /label=Ad5 left ITR
                     /note="Ad5 left ITR"
                     /note="ITR"
     promoter        67..75
                     /note="ATF binding site"
     misc_feature    243..251
                     /function="Ad5 genome packaging"
                     /note="A1"
                     /note="- A1 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    263..272
                     /function="Ad5 genome packaging"
                     /note="A2"
                     /note="- A2 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    305..314
                     /function="Ad5 DNA packaging"
                     /note="A3"
                     /note="- A3 repeat
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    316..324
                     /function="Ad5 DNA packaging"
                     /note="A4"
                     /note="- A4 repeat
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    340..349
                     /function="Ad5 DNA packaging"
                     /note="A5"
                     /note="- A5 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    362..370
                     /function="Ad5 DNA packaging"
                     /note="A6"
                     /note="- A6 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    370..378
                     /function="Ad5 DNA packaging"
                     /note="A7"
                     /note="- A7 repeat
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     CDS             612..1034
                     /codon_start=1
                     /gene="pIX"
                     /product="Ad5 pIX"
                     /label=Ad5 pIX
                     /note="Ad5 pIX"
                     /db_xref="GI:<html><body>33465835</body></html>"
                     /protein_id="AAQ19288.1"
                     /translation="MSTNSFDGSIVSSYLTTRMPPWAGVRQNVMGSSIDGRPVLPANST
                     TLTYETVSGTPLETAASAAASAAAATARGIVTDFAFLSPLASSAASRSSARDDKLTALL
                     AQLDSLTRELNVVSQQLLDLRQQVSALKASSPPNAV"
     rep_origin      complement(1110..1698)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1873..2688)
                     /codon_start=1
                     /product="Kanamycin-R"
                     /label=Kanamycin-R
                     /note="Kanamycin-R"
                     /note="origin: Tn903"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_signal     2751..2998
                     /label=Cos site
                     /note="Cos site"
                     /note="phage lambda COS site"
ORIGIN
        1 taacatcatc aataatatac cttattttgg attgaagcca atatgataat gagggggtgg
       61 agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg acgtagtagt gtggcggaag
      121 tgtgatgttg caagtgtggc ggaacacatg taagcgacgg atgtggcaaa agtgacgttt
      181 ttggtgtgcg ccggtgtaca caggaagtga caattttcgc gcggttttag gcggatgttg
      241 tagtaaattt gggcgtaacc gagtaagatt tggccatttt cgcgggaaaa ctgaataaga
      301 ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg taatatttgt ctagggccgc
      361 ggggactttg accgtttacg tggagactcg cccaggtgtt tttctcaggt gttttccgcg
      421 ttccgggtca aagttggcgt tttagatctt ctagacccgg gagcggccgc tgtcgacctg
      481 caggatccga attcgatatc actagtggta ccagtactga aatgtgtggg cgtggcttaa
      541 gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag
      601 ccgccgccgc catgagcacc aactcgtttg atggaagcat tgtgagctca tatttgacaa
      661 cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat gggctccagc attgatggtc
      721 gccccgtcct gcccgcaaac tctactacct tgacctacga gaccgtgtct ggaacgccgt
      781 tggagactgc agcctccgcc gccgcttcag ccgctgcagc caccgcccgc gggattgtga
      841 ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc ttcccgttca tccgcccgcg
      901 atgacaagtt gacggctctt ttggcacaat tggattcttt gacccgggaa cttaatgtcg
      961 tttctcagca gctgttggat ctgcgccagc aggtttctgc cctgaaggct tcctcccctc
     1021 ccaatgcggt ttaacggtcc gggccagagt ggccgagcaa aaggccagca aaaggccagg
     1081 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1141 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1201 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1261 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1321 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1381 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1441 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1501 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1561 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1621 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1681 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1741 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcggccaa
     1801 gaaggccaga gtggcagaga ctgcattcga aaacgtttga attgataatt attatcattt
     1861 gcgggtcaat tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     1921 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     1981 gaggcagttc cataggattg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     2041 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     2101 atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg
     2161 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     2221 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca
     2281 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     2341 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag
     2401 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     2461 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     2521 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     2581 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     2641 atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt
     2701 attactgttt atgtaagcag acagttttat tgttcatgcg aaaacgtttg aattgataat
     2761 tattatcatt tgcgggtcct ttccggcgat ccgccttgtt acggggcggc gacctcgcgg
     2821 gttttcgcta tttatgaaaa ttttccggtt taaggcgttt ccgttcttct tcgtcataac
     2881 ttaatgtttt tatttaaaat accctctgaa aagaaaggaa acgacagctg aaagcgagct
     2941 ttttggcctc tgtcgtttcc tttctctgtt tttgtccgtg gaatgaacaa tggaagtcgg
     3001 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagcgata
     3061 tttaaattaa t
//
