LOCUS       Exported                6036 bp ds-DNA     circular SYN 22-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAd1127-03
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Xavier
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 22, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   4..106
                     /label=Ad5 left ITR
                     /note="Ad5 left ITR"
                     /note="ITR"
     promoter        67..75
                     /note="ATF binding site"
     misc_signal     194..344
                     /note="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     misc_feature    243..251
                     /function="Ad5 genome packaging"
                     /note="A1"
                     /note="- A1 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    263..272
                     /function="Ad5 genome packaging"
                     /note="A2"
                     /note="- A2 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    305..314
                     /function="Ad5 DNA packaging"
                     /note="A3"
                     /note="- A3 repeat
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    316..324
                     /function="Ad5 DNA packaging"
                     /note="A4"
                     /note="- A4 repeat
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    340..349
                     /function="Ad5 DNA packaging"
                     /note="A5"
                     /note="- A5 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     gene            436..1600
                     /product="- The primary E1A transcript is processed by 
                     differential splicing to yield 5 distinct mRNAs: 13S, 12S, 
                     11S, 10S, and 9 S, which respectively code for proteins 
                     with 289 residues (289R), 243R, 217R, 171R, and 55R, all of
                     which are detectable in vivo with the exception of the 9S 
                     product which has only been detected in vitro. 
                     (http://publish.uwo.ca/~jmymryk/E1A/intro.html)
                     - The 13S and 12S E1A mRNAs are the most abundant at early 
                     times during infection.  The 9S nRNA is the most abundant 
                     at late times.  The 11S and 10S are minor species that 
                     become more abundant at late times after the infection. 
                     (http://publish.uwo.ca/~jmymryk/E1A/intro.html)"
                     /function="- The  E1A proteins, especially the 289R, 
                     augment E1A gene transcription to high levels, and also 
                     stimulate promoter activities of other viral genes, 
                     including the promoter for the E1B, E1A, E3, and E4 early 
                     genes, as well as the major late promoter (Yoshida et al, 
                     1995, The molecular Repertoire of Adenovirus III, 
                     pp113-130)
                     - E1A proteins reprogram cell growth to provide an optimal 
                     environment for viral replication.  They interact with a 
                     variety of cellular proteins, including transcriptional 
                     co-activators such as the CREB-binding protein (CBP) and 
                     p300, the p300/CBP Associated Factor (pCAF), and the 
                     transcriptional co-repressors CtBP and BS69, the 
                     TAT-binding (TBP) protein component of the general 
                     transcriptional machinery, pRB and related p130 and p107, 
                     the cyclin-dependent kinase inhibitor p27, SUMO conjugase 
                     UBC9 and proteasome components (Avvakumov et al, 2004, 
                     Virology 329: 477-492)"
                     /note="Ad5 E1A"
                     /note="- first gene expressed after infection of the host 
                     cell
                     - Ad5 E1A = Ad2 E1A"
     regulatory      436..443
                     /regulatory_class="TATA_box"
                     /gene="E1A"
                     /note="E1A TATA Box"
     CDS             join(528..915,1094..1513)
                     /codon_start=1
                     /product="Ad5 E1a"
                     /label=Ad5 E1a
                     /note="Ad5 E1a"
                     /translation="MRHIICHGGVITEEMAASLLDQLIEEVLADNLPPPSHFEPPTLHE
                     LYDLDVTAPEDPNEEAVSQIFPDSVMLAVQEGIDLLTFPPAPGSPEPPHLSRQPEQPEQ
                     RALGPVSMPNLVPEVIDLTCHEAGFHGQWVIEWWVWCGNFFFNFYSFVV*RILYCDFFK
                     RSCV*T*A*ARARTGACKTYPPS*NGACYPETPDITCV*RMQ**YG*L*LRSF*HTS*D
                     TPGGPAVPH*TSCRESWWASPGCGMYRGLA*RAWATFGLEL*TPQAI"
     CDS             join(528..942,1197..1512)
                     /codon_start=1
                     /gene="E1A"
                     /product="E1A 243R = 27 kDa protein"
                     /note="Ad5 E1A 243R"
                     /note="derived from the E1A 12S mRNA"
                     /db_xref="GI:<html><body>33465832</body></html>"
                     /protein_id="AAQ19285.1"
                     /translation="MRHIICHGGVITEEMAASLLDQLIEEVLADNLPPPSHFEPPTLHE
                     LYDLDVTAPEDPNEEAVSQIFPDSVMLAVQEGIDLLTFPPAPGSPEPPHLSRQPEQPEQ
                     RALGPVSMPNLVPEVIDLTCHEAGFPPSDDEDEEGPVSEPEPEPEPEPEPARPTRRPKM
                     APAILRRPTSPVSRECNSSTDSCDSGPSNTPPEIHPVVPLCPIKPVAVRVGGRRQAVEC
                     IEDLLNEPGQPLDLSCKRPRP"
     protein_bind    582..611
                     /bound_moiety="CREB-binding protein (CBP) and p300"
                     /note="CBP"
                     /note="Avvakumov et al, 2004, Virology 329:477-492 "
     misc_feature    651..743
                     /note="CR1"
                     /note="- E1A Conserved Region 1 in human and simian 
                     adenovirus serotypes
                     - overlaps with the weakest of two Rb-binding sites 
                     (HFEPPTLHELYDLDVTAP, aa 37-54), the strongest site being 
                     located in CR2
                     - contains one of three binding sites for CREB-binding 
                     protein (CBP)/p300: FPDSVML (consensus FXE/DXXXL). The two 
                     other CBP binding sites are located at the N-terminal end 
                     of E1A. 
                     (Avvakumov et al, Virology 2004, 329:477-492)"
     protein_bind    723..743
                     /bound_moiety="TRAM motif located in CH3 region of the 
                     CREB-binding protein (CBP)"
                     /note="CBP"
                     /note="O'Connor et al, 1999.  J. Virol 73: 3574-3581"
     misc_feature    870..938
                     /gene="E1A"
                     /function="Mediates binding of E1A proteins to pRB family 
                     proteins (pRB, p107, p130), dissociating them from the E2F 
                     transcription factor.  E2F can then activate cellular genes
                     involved in cell cycle and DNA synthesis and also the E2 
                     viral genes."
                     /note="CR2"
                     /note="- E1A Conserved Region 2 among human and simian 
                     adenovirus serotypes
                     - a.p. 122-126: consensus sequence LXCXE (where X is 
                     variable), which is the strongest of 2 binding sites for Rb
                     protein
                     - a.p. 113-117: consensus sequence PXLXP, which binds to 
                     transcriptional co-repressor BS69
                     (Avvakumov et al, Virology 2004, 329:477-92)."
     misc_feature    891..914
                     /note="D24"
                     /note="- 24-bp deletion (= aa LTCHEAGF), which encompasses 
                     the Rb-binding consensus site LXCXE (where X is variable). 
                     LXCXE is absolutely conserved in all 34 E1A sequences 
                     analyzed by Avvakumov (Virology 2004, 329: 477-492).  In 
                     Ad5, the phenylalanine residue located 3 aa downstream from
                     the LXCXE motif could interact with a hydrophobic pocket on
                     Rb.
                     - the 24-bp deletion helps targeting oncolytic adenoviruses
                     to tumor cells characterized by abnormal Rb control (Fueyo 
                     et al, Oncogene 200, 19:2-12)."
     intron          943..1196
                     /gene="E1A"
                     /note="E1A 12S"
     misc_feature    join(957..1080,1197..1216)
                     /note="CR3"
                     /note="- E1A Conserved Region 3 in human and simian 
                     adenovirus serotypes
                     - Cysteins at positions 154, 157, 171, and 174 form the 
                     zinc finger and are absolutely conserved in 34 human and 
                     simian adenovirus strains analyzed by Avvakumov et al 
                     (Virology 2004, 329:477-92)."
     intron          1081..1196
                     /gene="E1A"
                     /note="E1A 13S"
     misc_feature    1361..1507
                     /note="CR4"
                     /note="- E1A Conserved Region 4 in human and simian 
                     adenovirus serotypes
                     - Contains 2 binding sites for the transcriptional 
                     co-repressor CtBP
                     - Overlaps with the C-terminal Nuclear Localization Signal 
                     KRPRP
                     - The role of most of the conserved aa in CR4 is unknown
                     (Avvakumov et al, Virology 2004, 329:477-92)"
     misc_signal     1496..1510
                     /note="E1A NLS"
                     /note="- conventional, constitutively active E1A nuclear 
                     localization signal
                     - There is a second, non-conventional, 
                     developmentally-regulated NLS with the consensus: 
                     FV(X)7-20MXSLXYM(X)4MF, located in CR3, thus unique for the
                     larger 289R protein"
     regulatory      1622..1630
                     /regulatory_class="GC_signal"
                     /function="Binding site for SP1 transcription factor"
                     /note="SP1 binding site"
                     /note="-The presence of the GC box and TATA box is 
                     sufficient for high level of E1B gene transcription (Parks 
                     et al, J. Virol. 1988, 62:54-67)."
     gene            1640..3477
                     /gene="E1B"
                     /note="E1B gene"
     CDS             1682..2212
                     /codon_start=1
                     /product="Ad5 E1b 19K"
                     /label=Ad5 E1b 19K
                     /note="Ad5 E1b 19K"
                     /translation="MEAWECLEDFSAVRNLLEQSSNSTSWFWRFLWGSSQAKLVCRIKE
                     DYKWEFEELLKSCGELFDSLNLGHQALFQEKVIKTLDFSTPGRAAAAVAFLSFIKDKWS
                     EETHLSGGYLLDFLAMHLWRAVVRHKNRLLLLSSVRPAIIPTEEQQQQQEEARRRRQEQ
                     SPWNPRAGLDPRE"
     CDS             1987..3477
                     /codon_start=1
                     /product="Ad5 E1b 55K"
                     /label=Ad5 E1b 55K
                     /note="Ad5 E1b 55K"
                     /translation="MERRNPSERGVPAGFSGHASVESGCETQESPATVVFRPPGDNTDG
                     GAAAAAGGSQAAAAGAEPMEPESRPGPSGMNVVQVAELYPELRRILTITEDGQGLKGVK
                     RERGACEATEEARNLAFSLMTRHRPECITFQQIKDNCANELDLLAQKYSIEQLTTYWLQ
                     PGDDFEEAIRVYAKVALRPDCKYKISKLVNIRNCCYISGNGAEVEIDTEDRVAFRCSMI
                     NMWPGVLGMDGVVIMNVRFTGPNFSGTVFLANTNLILHGVSFYGFNNTCVEAWTDVRVR
                     GCAFYCCWKGVVCRPKSRASIKKCLFERCTLGILSEGNSRVRHNVASDCGCFMLVKSVA
                     VIKHNMVCGNCEDRASQMLTCSDGNCHLLKTIHVASHSRKAWPVFEHNILTRCSLHLGN
                     RRGVFLPYQCNLSHTKILLEPESMSKVNLNGVFDMTMKIWKVLRYDETRTRCRPCECGG
                     KHIRNQPVMLDVTEELRPDHLVLACTRAEFGSSDEDTD"
     CDS             3577..3999
                     /codon_start=1
                     /product="Ad5 pIX"
                     /label=Ad5 pIX
                     /note="Ad5 pIX"
                     /translation="MSTNSFDGSIVSSYLTTRMPPWAGVRQNVMGSSIDGRPVLPANST
                     TLTYETVSGTPLETAASAAASAAAATARGIVTDFAFLSPLASSAASRSSARDDKLTALL
                     AQLDSLTRELNVVSQQLLDLRQQVSALKASSPPNAV"
     misc_feature    complement(4075..4663)
                     /product="pUC19 Ori"
                     /label=pUC19 Ori
                     /note="pUC19 Ori"
     CDS             complement(4838..5653)
                     /codon_start=1
                     /product="Kanamycin-R"
                     /label=Kanamycin-R
                     /note="Kanamycin-R"
                     /note="origin: Tn903"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_signal     5716..5963
                     /label=Cos site
                     /note="Cos site"
                     /note="phage lambda COS site"
ORIGIN
        1 taacatcatc aataatatac cttattttgg attgaagcca atatgataat gagggggtgg
       61 agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg acgtagtagt gtggcggaag
      121 tgtgatgttg caagtgtggc ggaacacatg taagcgacgg atgtggcaaa agtgacgttt
      181 ttggtgtgcg ccggtgtaca caggaagtga caattttcgc gcggttttag gcggatgttg
      241 tagtaaattt gggcgtaacc gagtaagatt tggccatttt cgcgggaaaa ctgaataaga
      301 ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg taatatttgt ctagggagat
      361 cttctagacc cgggagcggc cgctgtcgac ctgcaggatc cgaattcgat atcactagtg
      421 gtaccacgtg tagtgtattt atacccggtg agttcctcaa gaggccactc ttgagtgcca
      481 gcgagtagag ttttctcctc cgagccgctc cgacaccggg actgaaaatg agacatatta
      541 tctgccacgg aggtgttatt accgaagaaa tggccgccag tcttttggac cagctgatcg
      601 aagaggtact ggctgataat cttccacctc ctagccattt tgaaccacct acccttcacg
      661 aactgtatga tttagacgtg acggcccccg aagatcccaa cgaggaggcg gtttcgcaga
      721 tttttcccga ctctgtaatg ttggcggtgc aggaagggat tgacttactc acttttccgc
      781 cggcgcccgg ttctccggag ccgcctcacc tttcccggca gcccgagcag ccggagcaga
      841 gagccttggg tccggtttct atgccaaacc ttgtaccgga ggtgatcgat cttacctgcc
      901 acgaggctgg ctttccaccc agtgacgacg aggatgaaga gggtgaggag tttgtgttag
      961 attatgtgga gcaccccggg cacggttgca ggtcttgtca ttatcaccgg aggaatacgg
     1021 gggacccaga tattatgtgt tcgctttgct atatgaggac ctgtggcatg tttgtctaca
     1081 gtaagtgaaa attatgggca gtgggtgata gagtggtggg tttggtgtgg taattttttt
     1141 tttaattttt acagttttgt ggtttaaaga attttgtatt gtgatttttt taaaaggtcc
     1201 tgtgtctgaa cctgagcctg agcccgagcc agaaccggag cctgcaagac ctacccgccg
     1261 tcctaaaatg gcgcctgcta tcctgagacg cccgacatca cctgtgtcta gagaatgcaa
     1321 tagtagtacg gatagctgtg actccggtcc ttctaacaca cctcctgaga tacacccggt
     1381 ggtcccgctg tgccccatta aaccagttgc cgtgagagtt ggtgggcgtc gccaggctgt
     1441 ggaatgtatc gaggacttgc ttaacgagcc tgggcaacct ttggacttga gctgtaaacg
     1501 ccccaggcca taaggtgtaa acctgtgatt gcgtgtgtgg ttaacgcctt tgtttgctga
     1561 atgagttgat gtaagtttaa taaagggtga gataatgttt aacttgcatg gcgtgttaaa
     1621 tggggcgggg cttaaagggt atataatgcg ccgtgggcta atcttggtta catctgacct
     1681 catggaggct tgggagtgtt tggaagattt ttctgctgtg cgtaacttgc tggaacagag
     1741 ctctaacagt acctcttggt tttggaggtt tctgtggggc tcatcccagg caaagttagt
     1801 ctgcagaatt aaggaggatt acaagtggga atttgaagag cttttgaaat cctgtggtga
     1861 gctgtttgat tctttgaatc tgggtcacca ggcgcttttc caagagaagg tcatcaagac
     1921 tttggatttt tccacaccgg ggcgcgctgc ggctgctgtt gcttttttga gttttataaa
     1981 ggataaatgg agcgaagaaa cccatctgag cggggggtac ctgctggatt ttctggccat
     2041 gcatctgtgg agagcggttg tgagacacaa gaatcgcctg ctactgttgt cttccgtccg
     2101 cccggcgata ataccgacgg aggagcagca gcagcagcag gaggaagcca ggcggcggcg
     2161 gcaggagcag agcccatgga acccgagagc cggcctggac cctcgggaat gaatgttgta
     2221 caggtggctg aactgtatcc agaactgaga cgcattttga caattacaga ggatgggcag
     2281 gggctaaagg gggtaaagag ggagcggggg gcttgtgagg ctacagagga ggctaggaat
     2341 ctagctttta gcttaatgac cagacaccgt cctgagtgta ttacttttca acagatcaag
     2401 gataattgcg ctaatgagct tgatctgctg gcgcagaagt attccataga gcagctgacc
     2461 acttactggc tgcagccagg ggatgatttt gaggaggcta ttagggtata tgcaaaggtg
     2521 gcacttaggc cagattgcaa gtacaagatc agcaaacttg taaatatcag gaattgttgc
     2581 tacatttctg ggaacggggc cgaggtggag atagatacgg aggatagggt ggcctttaga
     2641 tgtagcatga taaatatgtg gccgggggtg cttggcatgg acggggtggt tattatgaat
     2701 gtaaggttta ctggccccaa ttttagcggt acggttttcc tggccaatac caaccttatc
     2761 ctacacggtg taagcttcta tgggtttaac aatacctgtg tggaagcctg gaccgatgta
     2821 agggttcggg gctgtgcctt ttactgctgc tggaaggggg tggtgtgtcg ccccaaaagc
     2881 agggcttcaa ttaagaaatg cctctttgaa aggtgtacct tgggtatcct gtctgagggt
     2941 aactccaggg tgcgccacaa tgtggcctcc gactgtggtt gcttcatgct agtgaaaagc
     3001 gtggctgtga ttaagcataa catggtatgt ggcaactgcg aggacagggc ctctcagatg
     3061 ctgacctgct cggacggcaa ctgtcacctg ctgaagacca ttcacgtagc cagccactct
     3121 cgcaaggcct ggccagtgtt tgagcataac atactgaccc gctgttcctt gcatttgggt
     3181 aacaggaggg gggtgttcct accttaccaa tgcaatttga gtcacactaa gatattgctt
     3241 gagcccgaga gcatgtccaa ggtgaacctg aacggggtgt ttgacatgac catgaagatc
     3301 tggaaggtgc tgaggtacga tgagacccgc accaggtgca gaccctgcga gtgtggcggt
     3361 aaacatatta ggaaccagcc tgtgatgctg gatgtgaccg aggagctgag gcccgatcac
     3421 ttggtgctgg cctgcacccg cgctgagttt ggctctagcg atgaagatac agattgaagt
     3481 actgaaatgt gtgggcgtgg cttaagggtg ggaaagaata tataaggtgg gggtcttatg
     3541 tagttttgta tctgttttgc agcagccgcc gccgccatga gcaccaactc gtttgatgga
     3601 agcattgtga gctcatattt gacaacgcgc atgcccccat gggccggggt gcgtcagaat
     3661 gtgatgggct ccagcattga tggtcgcccc gtcctgcccg caaactctac taccttgacc
     3721 tacgagaccg tgtctggaac gccgttggag actgcagcct ccgccgccgc ttcagccgct
     3781 gcagccaccg cccgcgggat tgtgactgac tttgctttcc tgagcccgct tgcaagcagt
     3841 gcagcttccc gttcatccgc ccgcgatgac aagttgacgg ctcttttggc acaattggat
     3901 tctttgaccc gggaacttaa tgtcgtttct cagcagctgt tggatctgcg ccagcaggtt
     3961 tctgccctga aggcttcctc ccctcccaat gcggtttaac ggtccgggcc agagtggccg
     4021 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4081 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4141 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4201 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4261 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4321 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4381 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4441 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4501 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4561 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4621 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4681 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4741 attatcaaaa aggatcttcg gccaagaagg ccagagtggc agagactgca ttcgaaaacg
     4801 tttgaattga taattattat catttgcggg tcaattctta gaaaaactca tcgagcatca
     4861 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     4921 tctgtaatga aggagaaaac tcaccgaggc agttccatag gattgcaaga tcctggtatc
     4981 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     5041 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     5101 gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     5161 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     5221 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     5281 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     5341 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     5401 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     5461 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     5521 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     5581 catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac gtttcccgtt
     5641 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     5701 atgcgaaaac gtttgaattg ataattatta tcatttgcgg gtcctttccg gcgatccgcc
     5761 ttgttacggg gcggcgacct cgcgggtttt cgctatttat gaaaattttc cggtttaagg
     5821 cgtttccgtt cttcttcgtc ataacttaat gtttttattt aaaataccct ctgaaaagaa
     5881 aggaaacgac agctgaaagc gagctttttg gcctctgtcg tttcctttct ctgtttttgt
     5941 ccgtggaatg aacaatggaa gtcggcctcg tgatacgcct atttttatag gttaatgtca
     6001 tgataataat ggtttcttag cgatatttaa attaat
//
