LOCUS       Exported                2984 bp ds-DNA     circular SYN 16-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAd1127-02
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 16, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     repeat_region   4..106
                     /label=Ad5 left ITR
                     /note="Ad5 left ITR"
                     /note="ITR"
     promoter        67..75
                     /note="ATF binding site"
     misc_feature    243..251
                     /function="Ad5 genome packaging"
                     /note="A1"
                     /note="- A1 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    263..272
                     /function="Ad5 genome packaging"
                     /note="A2"
                     /note="- A2 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    305..314
                     /function="Ad5 DNA packaging"
                     /note="A3"
                     /note="- A3 repeat
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    316..324
                     /function="Ad5 DNA packaging"
                     /note="A4"
                     /note="- A4 repeat
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     misc_feature    340..349
                     /function="Ad5 DNA packaging"
                     /note="A5"
                     /note="- A5 repeat
                     - consensus sequence: 5'-TTTG-N8-CG-3'
                     - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35"
     CDS             525..947
                     /codon_start=1
                     /gene="pIX"
                     /product="Ad5 pIX"
                     /label=Ad5 pIX
                     /note="Ad5 pIX"
                     /db_xref="GI:<html><body>33465835</body></html>"
                     /protein_id="AAQ19288.1"
                     /translation="MSTNSFDGSIVSSYLTTRMPPWAGVRQNVMGSSIDGRPVLPANST
                     TLTYETVSGTPLETAASAAASAAAATARGIVTDFAFLSPLASSAASRSSARDDKLTALL
                     AQLDSLTRELNVVSQQLLDLRQQVSALKASSPPNAV"
     rep_origin      complement(1023..1611)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1786..2601)
                     /codon_start=1
                     /product="Kanamycin-R"
                     /label=Kanamycin-R
                     /note="Kanamycin-R"
                     /note="origin: Tn903"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_signal     2664..2911
                     /label=Cos site
                     /note="Cos site"
                     /note="phage lambda COS site"
ORIGIN
        1 taacatcatc aataatatac cttattttgg attgaagcca atatgataat gagggggtgg
       61 agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg acgtagtagt gtggcggaag
      121 tgtgatgttg caagtgtggc ggaacacatg taagcgacgg atgtggcaaa agtgacgttt
      181 ttggtgtgcg ccggtgtaca caggaagtga caattttcgc gcggttttag gcggatgttg
      241 tagtaaattt gggcgtaacc gagtaagatt tggccatttt cgcgggaaaa ctgaataaga
      301 ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg taatatttgt ctagggagat
      361 cttctagacc cgggagcggc cgctgtcgac ctgcaggatc cgaattcgat atcactagtg
      421 gtaccagtac tgaaatgtgt gggcgtggct taagggtggg aaagaatata taaggtgggg
      481 gtcttatgta gttttgtatc tgttttgcag cagccgccgc cgccatgagc accaactcgt
      541 ttgatggaag cattgtgagc tcatatttga caacgcgcat gcccccatgg gccggggtgc
      601 gtcagaatgt gatgggctcc agcattgatg gtcgccccgt cctgcccgca aactctacta
      661 ccttgaccta cgagaccgtg tctggaacgc cgttggagac tgcagcctcc gccgccgctt
      721 cagccgctgc agccaccgcc cgcgggattg tgactgactt tgctttcctg agcccgcttg
      781 caagcagtgc agcttcccgt tcatccgccc gcgatgacaa gttgacggct cttttggcac
      841 aattggattc tttgacccgg gaacttaatg tcgtttctca gcagctgttg gatctgcgcc
      901 agcaggtttc tgccctgaag gcttcctccc ctcccaatgc ggtttaacgg tccgggccag
      961 agtggccgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1021 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1081 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1141 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1201 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1261 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1321 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1381 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1441 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1501 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1561 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1621 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1681 gtcatgagat tatcaaaaag gatcttcggc caagaaggcc agagtggcag agactgcatt
     1741 cgaaaacgtt tgaattgata attattatca tttgcgggtc aattcttaga aaaactcatc
     1801 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     1861 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga ttgcaagatc
     1921 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     1981 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     2041 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     2101 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     2161 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     2221 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     2281 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     2341 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     2401 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     2461 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     2521 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt
     2581 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     2641 tattgttcat gcgaaaacgt ttgaattgat aattattatc atttgcgggt cctttccggc
     2701 gatccgcctt gttacggggc ggcgacctcg cgggttttcg ctatttatga aaattttccg
     2761 gtttaaggcg tttccgttct tcttcgtcat aacttaatgt ttttatttaa aataccctct
     2821 gaaaagaaag gaaacgacag ctgaaagcga gctttttggc ctctgtcgtt tcctttctct
     2881 gtttttgtcc gtggaatgaa caatggaagt cggcctcgtg atacgcctat ttttataggt
     2941 taatgtcatg ataataatgg tttcttagcg atatttaaat taat
//
