Experts in Adenovirus Vectors

Ad408

Image
Ad5.CMV-GFP_AD106-S

Ad408

Replication-deficient Ad5 with CMV-GFP expression cassette in place of the E1 region

  • Purified by double CsCl banding
  • A total of 5 x 1011 VP will be provided in 100 µL aliquots.  The number of vials you will receive will depend on the virus concentration.  Contact us for more information.
$440.00
SKU
AD106-S
Size
5 x 10^11 VP
Description

Ad408 is a replication-deficient Ad5 adenovirus containing a GFP expression cassette in place of the E1 region, oriented towards the left ITR. The GFP coding sequence is Dasher-GFP, an IP-free version of Aequorea GFP engineered by ATUM Inc.  It is under the control of a human cytomegalovirus (CMV) promoter and a bovine growth hormone (bGH) polyadenylation signal. The E3 region has a 1.9 kb XbaI deletion that encompasses the 3’ end of the E3 6.7K gene, the entire E3 gp19K, ADP, RIDα, RIDβ genes, and the 5’ end of the E3 14.7K gene.

FAQs

 

Which formulation do you use for purified adenovirus vectors?

We use GTS buffer (2.5 % glycerol (w/v), 25 mM NaCl in 20 mM Tris, pH 8.0) (Hoganson et al, 2002. Bioprocessing J. 1:43-48).  The buffer provides stability for at least 4 freeze/thaw cycles, and for at least a year when it is frozen at -80 °C (Gilbert et al, 2004. Mol Ther 9, S294–S294).

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