Experts in Adenovirus Vectors

Cosmid Construction Kit-2

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Cosmid Construction Kit-2

  • Sufficient for constructing at least 10 vectors (that is only $10.50 per construct)
  • Reagents are automatically added to your order when you buy the AdenoQuick2.0 kits
  • Contents:
    • maltose/MgSO4(# R-1023)
    • SM medium (# R-1004)
    • λ packaging extract (#R-1005)
    • E. coli OD101 glycerol stock (#R-1006)
    • Amp/Kan antibiotic mix (#R-1007)
    • SfiI enzyme (#R-1008)
    • SfiI 10x buffer (#R-1009)
  • Storage: -20 ºC for SfiI enzyme and -70 ºC for lambda packaging extract
$105.00
SKU
R-1002
Size
10 reactions
Description

The Cosmid Construction Kit -2  contains most of the reagents necessary to construct AdenoQuick2.0 cosmids:

  • The SfiI restriction enzyme is used to digest the 4 shuttle plasmids before ligating them to each other.
  • OD101 is the E. coli strain used to construct and amplify adenovirus cosmid DNAs.
  • Maltose and MgSO4 are added to the E. coli culture to induce expression of the receptor for phage λ
  • The λ packaging extract is used to package the DNA ligation products into the phage λ heads.
  • The SM medium is used to stop the packaging reaction at the end of the 30-minutes incubation period.
  • The Amp/Kan antibiotic mix will be used to select for colonies containing the adenovirus cosmid.

The reagents that the user needs to provide are:

  • T4 DNA ligase
  • Chloroform
  • LB Lennox (liquid and petri dishes)

The SfiI restriction enzyme must be stored at - 20 ºC.  The lambda packaging extract must be stored at - 70 ºC.

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